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| Registros recuperados: 50 | |
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| Robb, F; Muir, D; Davis, C. |
| Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems... |
| Tipo: Text |
Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf |
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| Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R. |
| 110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf |
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| Nicolas, Jean-louis; Ansquer, Dominique; Cochard, Jean-claude. |
| A bacterial strain belonging to the genus Vibrio was involved in a recurrent outbreak of disease in Manila clam Tapes philippinarum larvae at a commercial hatchery, This vibrio, named VTP for convenience, was not related to the known pathogenic vibrios and possessed unusual characteristics, Only Manila clam larvae and postlarvae were affected by the disease, Oyster and scallop larvae were not affected by vrp, Survival of vrp was found to be of short duration in seawater but even a very small number of cells (1 cell per 5 ml) could initiate the disease, VTP did not grow on thiosulfate-citratebile-sucrose agar and was resistant to chloramphenicol. An epidemiological study utilizing bioassays revealed the presence of VTP in adult bivalves and in the... |
| Tipo: Text |
Palavras-chave: Bacterial diseases; Clam culture; Larvae; Pathogenic bacteria; Tapes philippinarum; Vibrio. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00037/14872/12198.pdf |
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| Le Groumellec, M.; Haffner, Philippe; Martin, B.; Martin, C.. |
| Mass mortality associated with luminescence has been dbserved in sorne Thai and Ecuadorian penaeid shrimp hatcheries in recent years. Samples collected from hatcheries in these two different areas revealed luminescent sucrose-negative Vibrio strains in larVal rearing tanks where mortality occurred. Pathogenicity trials were carried out to identify the causative agents of mortality and to study the course of infection . . Results showed that strains isolated from Thai hatclleries were more virulent than Ecuadorian strains when tested on different larval stages of Penaeus monodon. The virulence of these strains was also estimated. Trials on other species ofpenaeid shrimp demonstrated the specifie action of Thai strains. Drug sensitivity patterns revealed a... |
| Tipo: Text |
Palavras-chave: Mass mortality; Bacterial; Vibrio; Hatcheries; Penaeus monodon; Shrimp. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/acte-6100.PDF |
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| Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique. |
| Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in... |
| Tipo: Text |
Palavras-chave: Larvae; Bivalve; Vibrio; Scallop. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf |
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| Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B. |
| Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida... |
| Tipo: Text |
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf |
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| Faury, Nicole; Saulnier, Denis; Thompson, F; Gay, Melanie; Swings, J; Leroux, Frederique. |
| Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T) =LMG 22240(T)=Clp 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine... |
| Tipo: Text |
Palavras-chave: Identification; Vibrio; Crasssotrea gigas; Ostreiculture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-591.pdf |
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| Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry. |
| Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and... |
| Tipo: Text |
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf |
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| Goarant, Cyrille; Ansquer, Dominique; Herlin, Jose; Domalain, David; Imbert, Frederic; De Decker, Sophie. |
| The Summer Syndrome is a new shrimp disease that has been affecting a shrimp growout farm in New Caledonia since end of 1997. It was recognized to be caused by a systemic vibriosis due to Vibrio nigripulchritudo. This new disease turned out almost immediately enzootic in the shrimp farm involved and has affected all its crops ever since. Since the year 2000, V. nigripulchritudo strains have been found in several shrimp farms, although Summer Syndrome is still limited to one particular area, affecting, since 2003, two adjoining farms. As part of a multidisciplinary research program, a high-frequency survey was carried out during the summer 2002-2003 in two shrimp farms: one affected farm and another one in which pathogenic strains could be isolated but... |
| Tipo: Text |
Palavras-chave: Epidemiology; Vibrio; Diseases and their control; Shrimp. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1110.pdf |
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| Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra. |
| In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF |
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| Goarant, Cyrille; Merien, F. |
| Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular... |
| Tipo: Text |
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf |
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| De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis. |
| Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and... |
| Tipo: Text |
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
| In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
| Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
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| De Decker, Sophie. |
| The bacteria of the Vibrio genus are one of the most abundant groups in the aquatic ecosystems. Two species of vibrios, Vibrio splendidus and V. Aestuarianus, have been associated, in some cases, to mortality in flat oyster Crassostrea gigas farms, most of the time during the summer period. This doctoral thesis studies the Vibrio-host interaction and their modulations according to the host's pathogenic virulence and its genetic, physiologic and immunitary parameters. These factors will be characterised by in vitro and in vivo approaches (experimental infection). The characterization of contrasted genetic families according to their sensitivity to these vibrios will enable the implementation of new improvement strategies in oyster farms (prophylactic... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres génétiques; Virulence; Oyster; Crassostrea gigas; Pathogène; Vibrio. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3431.pdf |
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| Saulnier, Denis; De Decker, Sophie; Haffner, Philippe. |
| Because Vibrio aestuarianus is known to cause serious infections in Pacific oyster Crassostrea gigas, a real-time PCR assay was developed targeting the dnaJ gene of this bacterium. Only V. aestuarianus strains isolated from C. gigas mortality events in different geographic areas and the reference strain tested positive, whereas no amplification products was obtained with type strains belonging to 23 other species of Vibrio. Sensitivity and reproducibility of the method were assessed using either seawater or oyster homogenate samples spiked with one V aestuarianus strain. All these samples were stored at -20 degrees C in order to mimic retrospective or grouped natural sample analysis without quantification bias due to prolonged freezing. Analysis of... |
| Tipo: Text |
Palavras-chave: V. aestuarianus; Vibrio; Taqman; Real time PCR; Pathogen; Oyster; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6447.pdf |
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| Registros recuperados: 50 | |
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